Spring Selection 2018

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closes 7 Jan 2018

Research Groups

Portrait Stephan Grill

Stephan Grill

Motor Systems

Previous and Current Research

Our group works in cellular biophysics. We are interested in how molecular machines, the workhorses of the cell, function and interact to form the dynamical processes that make up living organisms. The whole is greater than the sum of the parts – in this spirit we pursue an understanding of how molecular mechanisms give rise to specific behaviors that emerge at length and time scales relevant for processes at the cell biological level, using a combined experimental and theoretical approach.  Of special interest are large-scale actomyosin cytoskeletal dynamics and its relation to molecular mechanisms, and transcriptional pausing and its relation to molecular events that occur in the machinery that is involved. Our group is jointly appointed to the Max Planck Institute for the Physics of Complex Systems and the Max Planck Institute of Molecular Cell Biology and Genetics.

Cytoskeletal Systems
The main focus of our lab addresses the actomyosin cytoskeleton, where we investigate the physical basis of polarizing cortical flow in the early stages of life of the nematode Caenorhabditis elegans. Flow results from the ability of the actomyosin cortex to ‘contract’, a feature that emerges as a consequence of many molecules interacting; individual proteins cannot do this. We use imaging techniques to measure biophysical parameters such as flow velocity and alignment, and use UV laser ablation to measure cortical tension. We describe the mechanical basis of these cell biological events in terms of novel hydrodynamic descriptions of active materials. We use RNAi to perturb the function of different genes to characterize how they contribute to the mechanical behavior at cellular length and time scales.  A particular focus lies in developing approaches to understand the interplay between regulatory biochemistry and cytoskeletal mechanics that is central to the development of this nematode.

Transcriptional Systems
Here we study the molecular events that are at the heart of transcription, and how they give rise to more general types of behaviors such as transcriptional pausing and proofreading. We use single molecule techniques to follow the movement of individual RNA polymerase molecules, and make use of theoretical approaches to understand how distinct kinetic mechanisms relate their biophysical modes of operation to specific cellular roles and functions.

Stephan Grill research: figure
Fig.: Actomyosin cortex of a one cell stage Caenorhabditis elegans embryo cut with a pulsed UV laser along the blue line. Non-muscle-myosin 2 is visualized by GFP fluorescence (pre-ablation image in magenta, post-ablation image in green). Note that the material moves away from the line of cut (arrows), revealing that the material is under mechanical tension. Scale bar, 5 µm. Modified from Mayer et al., 2010.
Selected Publications

Mayer, Mirjam; Depken, Martin; Bois, Justin; Jülicher, Frank; Grill, Stephan W.:
Anisotropies in cortical tension reveal the physical basis of polarizing cortical flows.
Nature, 467, no. 7315, pp. 617-621 (2010)

Depken, Martin; Galburt, Eric A.; Grill, Stephan W.:
The origin of short transcriptional pauses.
Biophys. J., 96, no. 6, pp. 2189-2193, (2009)

Galburt, Eric A.; Grill, Stephan W.; Wiedmann, Anna; Lubkowska, Lucyna; Choy, Jason; Nogales, Eva; Kashlev, Mikhail; Bustamante, Carlos:
Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner.
Nature, 446, no. 7137, pp. 820-823, (2007)

Grill, Stephan W.; Kruse, Karsten; Jülicher, Frank:
Theory of mitotic spindle oscillations.
Phys. Rev. Lett., 94, no. 10, pp. 108104-108104, (2005)

Grill, Stephan W.; Howard, Jonathon; Schäffer, Erik; Stelzer, Ernst H K; Hyman, Anthony A.:
The distribution of active force generators controls mitotic spindle position.
Science, 301, no. 5632, pp. 518-521, (2003)

Grill, Stephan W.; Gönczy, Pierre; Stelzer, Ernst H K; Hyman, Anthony A.:
Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo.
Nature, 409, no. 6820, pp. 630-633, (2001)

CV

since 2013
Professor for Biophysics at the Biotechnology Center of the TU Dresden, Germany

2009
Acting Professor, Faculty of Physics and Earth Science, University of Leipzig, Germany

2006–2013
Group leader, jointly at the Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden and the Max-Planck-Institute for the Physics of Complex Systems, Dresden

2004–2005
Postdoctoral work at the Department of Physics, University of California in Berkeley, USA

2003
Postdoctoral work at MPI-CBG Dresden, Germany

1998–2002
PhD work at EMBL Heidelberg; PhD in Physics at the Technical University München, Germany

Contact

Biotechnological Center
TU Dresden
Tatzberg 47/49
01307 Dresden

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